Elimination of pleuropneumonia-like organisms from tissue culture.

Arginine Metabolism in Pleuropneumonia-like Organisms Isolated from Mammalian Cell Culture.

Schimke, Robert T. (National Institutes of Health, Bethesda, Md.) and Michael F. Barile. Arginine metabolism in pleuropneumonia-like organisms isolated from mammalian cell culture. J. Bacteriol. 86:195-206. 1963.-Arginine degradation is a significant metabolic process for pleuropneumonia-like organisms (PPLO; Mycoplasma) isolated from cell culture. The conversion of arginine to ornithine in PPL...

[Contamination of tissue cultures with pleuropneumonia-like organisms].

Antibiotic control of Mycoplasma in tissue culture.

Seven of eight strains of Mycoplasma (PPLO) were found to be sensitive to the deoxystreptamines, certain macrolides, and the tetracyclines. These antibiotics are relative noncytotoxic. Kanamycin and tetracycline were useful in eliminating PPLO (pleuropneumonia-like organisms) strain Squibb no. 1 from a HeLa cell line which was deliberately contaminated with PPLO. Repeated exposure of M. laidlaw...

The inhibitory action of agar on certain strains of pleuropneumonia-like organisms.

The pleuropneumonia-like organisms (PPLO) are recognized as a group of rather fastidious microorganisms. Various peptones, infusions, and serum supplements have been examined by many investigators relating to the development of a medium satisfactory for the in vitro cultivation of these organisms (Morton et al., 1951; Edward, 1947; and others). However, few reports of examinations of the agar i...

Use of Porous Cellulose Ester Membranes in the Primary Isolation and Size Determination of Pleuropneumonia-like Organisms.

Morowitz, Harold J. (Yale University, New Haven, Conn.), Mark E. Tourtellotte, and Mary E. Pollack. Use of porous cellulose ester membranes in the primary isolation and size determination of pleuropneumonia-like organisms. J. Bacteriol. 85:134-136. 1963.-Millipore filters were used in the primary isolation and sizing of a number of strains of pleuropneumonia-like organisms (PPLO). Maximal pore ...